Induction of petite yeast mutants by membrane-active agents
نویسندگان
چکیده
منابع مشابه
Induction of petite yeast mutants by membrane-active agents.
Ethanol proved to be a strong mutagenic agent of Saccharomyces mitochondrial DNA. Other active membrane solvents, such as tert-butanol, isopropanol, and sodium dodecyl sulfate, also turned out to be powerful petite mutation [rho-] inducers. Mutants defective in ergosterol synthesis (erg mutants) showed an extremely high frequency of spontaneous petite cells, suggesting that mitochondrial membra...
متن کاملThe induction of cytoplasmic petite mutants of Saccharomyces cerevisiae by hydrostatic pressure.
This study demonstrates that hydrostatic pressure is a potent inductive agent of the petite mutation in cultures of Saccharomyces cerevisiae. The inductive capacity of this mutagen is dependent on the magnitude and the duration of the pressure treatment. Furthermore, the extent of petite induction varies with the growth stage of the culture. Induction occurs in pressure-treated (1-4 X 1-(4) lbf...
متن کاملInduction of petite mutants in Saccharomyces cerevisiae by ICR-170 and their genetic characterization.
The strain employed was S288C, a genetically well-studied respiration-sufficient haploid that forms predominantly single cells and cells with one bud during log-phase growth and single cells in stationary phase. The respiratory genetic makeup of spontaneous and induced petites was determined by tetrad analysis and by complementation tests (PITTMAN et al. 1960; SHERMAN and EPHRUSSI 1962) using s...
متن کاملPetite mutation in yeast. II. Isolation of mutants containing mitochondrial deoxyribonucleic acid of reduced size.
A series of petite mutants of Saccharomyces cerevisiae, generated after treatment for various times with ethidium bromide, was isolated, and the mitochondrial deoxyribonucleic acid size for each member was estimated. It was found that, as the treatment time with ethidium bromide was increased, the mitochondrial deoxyribonucleic acid isolated from the petite series was increasingly reduced in size.
متن کاملComplementation in cytoplasmic petite mutants of yeast to form respiratory competent cells.
Complementation has been observed in cytoplasmic respiratory deficient yeast cells (petites) to yield respiratory competent diploids. This successful demonstration depended on the use of spontaneous petites of recent origin and on crosses involving all possible apirwise combinations between the many different petite isolates of opposite mating type. The possibility of deletion of a single uniqu...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1988
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.54.12.3126-3132.1988